Researcher’s Profile

Adam D. Griesemer, MD

Director, Large Animal Xenotransplantation Laboratory, Columbia Center for Translational Immunology
Overview: 

Allogeneic Studies

My laboratory is investigating the ability of ex-vivo expanded Tregs to enhance bone marrow engraftment and extend the duration of mixed chimerism. The induction of durable mixed chimerism without graft-versus-host disease should in turn lead to life-long donor-specific tolerance to any co-transplanted cells (islets) or solid organs (heart, liver, lung) from the donor. We are also collaborating with other investigators at the CCTI to study the ability of amnion-derived multipotent progenitor cells to enhance bone marrow engraftment in translational models as an alternate, and potentially synergistic, strategy to induce durable mixed chimerism.

We are also investigating the ability to translate the induction protocol that induces tolerance to simultaneously co-transplanted bone marrow and kidney grafts (despite only transient mixed chimerism developing) to combined bone marrow and liver grafts. In particular, we are investigating 1) the role of donor and recipient memory cells in mediating host-versus-graft and graft-versus-host responses; and 2) the role that inflammatory cytokines, particularly in the portal system, play in priming inflammatory and tolerogenic cellular responses.

The future goals of the laboratory include collaborating with the Chen lab in testing the ability of durable mixed chimerism to induce tolerance to organs that do not facilitate tolerance with the transient mixed chimerism approach, such as islets. We also plan to test the ability of modulating the T cell memory responses and the inflammatory environment induced after liver transplant to reliably induce rapid tolerance after liver transplants.

Xenogeneic Studies

The development of Gal-knockout swine removed the most significant target of natural antibodies from the surface of pig cells. However, low levels of non-Gal antibodies represent a barrier to long-term solid organ xenograft survival and xenogeneic bone marrow engraftment. Since pharmacological interventions have been unsuccessful in decreasing natural antibody levels, my lab is investigating the efficacy of Treg therapy in reducing anti-swine antibodies and facilitating tolerance induction to xenotransplants.

Current investigations include:

1. Studies on the optimal method of stimulating Tregs during expansion to generate donor-specific Tregs

2. Suppression of xenogeneic natural antibodies by donor-specific Tregs in preclinical models

3. Augmentation of xenogeneic chimerism using donor-specific Tregs

The future goals of the laboratory are to utilize donor-specific Tregs to control the natural anti-swine antibody response in translational swine-to-humanized mice and swine-to-nonhuman primate models. This in turn will facilitate the induction of xenogeneic chimerism that is expected to result in robust tolerance to donor solid organ transplants.

Lab Members: 
Paula Alonso-Guallart, DVM
Sulemon Chaudry, MD
Jeffery Stern, MD
Joshua Weiner, MD, Former Lab Member
Publications: 
Thome J, Bickham K, Ohmura Y, Kubota M, Matsuoka N, Gordon C, Granot T, Griesemer A, Lerner H, Kato T, and Farber D. Compartmentalization of infant T-cell differentiation and regulation in human tissues. Nature Medicine. Accepted - In Press
Soubrane O, Rougemont O, Kim K, Samstein B, Mamode N, Boillot O, Troisi R, Scatton O, Cauchy F, Lee S, Griesemer A, Ahmed Z, Clavien P, and Cherqui D. Laparoscopic Living Donor Left Lateral Sectionectomy: a New Standard Practice for Donor Hepatectomy. Annals of Surgery. Accepted – In Press.
Parekh M, Kluger M, Griesemer A, and Bentley-Hibbert S. Regenerative Liver Surgeries: The alphabet soup of emerging techniques. American Journal of Roentgenology. Accepted – In Press
Weiner J, Griesemer A, Island E, Lobritto S, Martinez M, Selvaggi G, Lefkowitch J, Velasco M, Tryphonopoulos P, Emond J, Tzakis A, Kato T. Long-Term Outcomes of Auxiliary Partial Orthotopic Liver Transplantation in Pre-Adolescent Children with Fulminant Hepatic Failure. Liver Transpl. 2015 Oct 19. PMID: 26479577
Matsuoka N, Weiner JI, Griesemer AD, Samstein BB, Zhao Y, et al. Ex Vivo Pancreaticoduodenectomy and Liver Autotransplantation for Pancreatic Head Tumor with Extensive Involvement of the Hepatoduodenal Ligament. Liver Transplantation. 2015; PMID: 26289503